Duties and Responsibilities: The AgCenter Biotechnology Laboratory (ABL) at the LSU AgCenter is seeking a highly motivated applicant with training and experience in the areas of Molecular Biology or Cell Biology. The successful candidate will be responsible for: (1) designing and cloning genes (2) transformation of bacteria and algae Chlamydomonas and analysis of transformants including PCR, RT-PCR, and Western blots (3) developing and executing new procedural routines and methods for analysis of proteins and other components in transgenic organisms (4) general laboratory maintenance including equipment and ordering supplies (5) managing activities of laboratory student workers. Knowledge of plant transformation is desirable. The successful candidate will be expected to perform experiments carefully, with minimal supervision; keep accurate lab records; organize and clean lab area. Good written and communication skills and the ability to work productively in a team-based research environment are required.
· Use in-depth knowledge of molecular biology for developing gene expression strategies in Chlamydomonas and bacteria.
· Use established transformation protocols to produce stable transformants of Chlamydomonas and express genes of interest.
· Use in-depth knowledge of molecular biology for developing and improving Chlamydomonas transformation protocols.
· Stay up to date and exchange important information about new developments that may be used to improve the outcome of the research project.
Job Knowledge and skills:
· Extensive knowledge of molecular biology methodologies
· Experience in cloning, gene design and construction
· Experience in transformation of microorganisms and preferably algae or plants
· Experience in protoplast isolation and culture
· Experience in performing biochemical bioassays
· Experience in media preparation, initiation of cultures, and maintenance of cultures
· Demonstrated ability to troubleshoot cloning and transformation protocols and methodologies
· Ability to work independently and as part of a team
· Good interpersonal skills
· Strong analytical and reasoning abilities
· Excellent communication skills; both written and verbal
· Willingness and capacity to learn and acquire relevant new skill sets
Qualification Requirements: a Masters of Science degree in Molecular Biology, Cell Biology, or a related field with at least two years of experience in a relevant field. Candidates with a Bachelors degree in the above fields with suitable practical experience will be considered.
LSu AgCenter